the 14kda protein molecule isolated from garlic suppresses indoleamine 2, 3-dioxygenase metabolites in mononuclear cells in vitro
نویسندگان
چکیده
a wide range of biological activities of garlic in vitro and in vivo have been verified including its antioxidant, antitumor and anti-inflammatory effects. indoleamine 2,3-dioxygenase (ido) is an enzyme widely distributed in mammals and is inducible preferentially by ifn-γ. ido degrades the essential amino acid tryptophan to form n-formyl kynurenine. in the present in vitro study, the modulatory effect of 14kda molecule isolated from garlic on ido induction was tested. cultures of mononuclear cells were exposed to 14kda garlic fraction. then, their proliferation responses and ido metabolites were measured. a significant down-regulatory effect of garlic on ido activity was found and also the proliferation responses of mononuclear cells increased. if these results are verified in vivo, an explanation will be provided on how garlic may interfere in ido induction, which paves the way for elucidating its specific therapeutic effect in preventing tumor progress.
منابع مشابه
The 14kDa protein molecule isolated from garlic suppresses indoleamine 2, 3-dioxygenase metabolites in mononuclear cells in vitro.
A wide range of biological activities of garlic in vitro and in vivo have been verified including its antioxidant, antitumor and anti-inflammatory effects. Indoleamine 2,3-dioxygenase (IDO) is an enzyme widely distributed in mammals and is inducible preferentially by IFN-gamma. IDO degrades the essential amino acid tryptophan to form N-formyl kynurenine. In the present in vitro study, the modul...
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indolemine 2, 3-dioxygenase (ido) is a cytosolic monomeric hemoprotein enzyme that catalyses tryptophan, the least available essential amino acid in the human body, to n-formylkynurenine, which in turn rapidly degrades to give kynurenine. ido is expressed in different tissues, especially and prominently in some subsets of antigen presenting cells (apcs) of lymphoid organs and also in the placen...
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Interferon (IFN)-induced tryptophan degradation, catalyzed by indoleamine 2,3-dioxygenase (IDO), has been shown to mediate antimicrobial activity in epithelial cells. IDO activity has also been augmented in peripheral blood mononuclear cells (PBMC) treated with IFN or interleukin-2 (IL-2). The effector cells in this population have now been further characterized. PBMCs were isolated from normal...
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عنوان ژورنال:
iranian journal of allergy, asthma and immunologyجلد ۷، شماره ۴، صفحات ۲۰۳-۲۰۸
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